Job Description

Job Summary:

Conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects and compiles data in a research lab; requires a practical understanding of a limited number of standard scientific techniques.



Specific Responsibilities:

Follows pre-defined protocols, policies, procedure, regulations, Good Clinical and Good Laboratory Practices and conducts standard and repetitive experiments and completes basic trouble-shooting techniques.
Sets-up, operates and maintains standard lab equipment.
Using an entry level of skill, completes the following activities:
Records test results and may maintain databases.
Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
Maintains inventory of reagents, mice and lab supplies
Molecular Biology:

DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting
PCR, RT-PCR
Plasmid preparation
Cloning, mapping and sequencing of DNA
Recombinant virus production and purification
Cellular Biology:

Cell culture, cell isolation
Functional studies
Transfection
Histology:

Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
Immunohistochemistry
Microscopy
Immunology:

Flow cytometry
ELISA, Elispot assay
Cytokine assay
Antibody production and purification



Animal:

Husbandry
Breeding and screening transgenic mice
Dissections
Blood collection
Surgical procedures
Database management of colony
Miscellaneous

Performs other duties as assigned.

Minimum Qualifications: (Education, experience, and any other certifications or clearances)

HS diploma or equivalent and some basic science courses is required.